High Performance Liquid Chromatography or HPLC, often referred to latterly as Liquid Chromatography or LC is a widely used technique for the analysis of soluble compounds. Depending on the column and detector used, a wide variety of compound types over a wide range of molecular weights can be assessed.
The TSG chromatography group has multiple HPLC systems all of which are equipped with binary or quarternary pumping systems allowing gradient programming.
All systems are fitted with robotic sampling devices allowing up to 100 samples to be analysed automatically.
Detection systems include ultraviolet (UV) detectors which are the most widely used in the field and are applicable to the analysis of the majority of pharmaceutical compounds and any compound which contains a UV chromophore. Single and multiple wavelength UV detectors are available.
Fluorescence detection allows for the very sensitive analysis of fluorescent compounds, often in complex matrices.
Refractive index (RI) is a universal detector for the analysis of compounds which do not have a UV chromophore such as sugars and polyols.
Evaporative light scattering detection is analogous to RI detection in that it is universal for semi or non-volatile compounds. However, it has markedly superior sensitivity.
Finally mass selective (MS) detection in the form of a single quadrupole is used to provide identification of targeted compounds in solution. More powerfully, by targeting selected ions in a compound sensitive quantitative analyses can be carried out on normally difficult matrices.